Journal: BMC Oral Health

Article Title: Chronic intermittent hypobaric hypoxia attenuates root resorption following delayed tooth replantation by upregulating OPG/RANKL signaling pathway

doi: 10.1186/s12903-026-08135-7

Figure Lengend Snippet: CIHH downregulates RANKL expression and increases the OPG/RANKL ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone

Article Snippet: Membranes were blocked with 5% skim milk and incubated with a mouse anti-OPG primary antibody (1:1000, Huabio, China) or a rabbit anti-RANKL primary antibody (1:1000, Huabio, China) overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: The expression levels of OPG , RANKL , RANK , and TLR4 mRNA in kidney tissues. RT-qPCR detected the mRNA expression levels of each gene. ( A ) OPG mRNA. ( B ) RANKL mRNA. ( C ) RANK mRNA. ( D ) TLR4 mRNA. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: The expression levels of OPG, RANKL, RANK, and TLR4 protein in kidney tissues. Western blot detected the protein expression levels in each group. ( A ) Representative Western blot results. ( B ) Relative level of OPG. ( C ) Relative level of RANKL. ( D ) Relative level of RANK. ( E ) Relative level of TLR4. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Expressing, Western Blot

Journal: BMC Nephrology

Article Title: Role of OPG/RANKL/RANK/TLR4 signaling pathway in sepsis-associated acute kidney injury

doi: 10.1186/s12882-024-03648-1

Figure Lengend Snippet: A schematic diagram illustrating the OPG/RANKL/RANK/TLR4 signaling pathway. RANKL competes with OPG for binding to RANK. Upon binding of RANKL to RANK, it triggers the interaction of RANK with TRAF6, leading to the suppression of the TLR4 signaling pathway, the activation of which is initiated upon the binding of LPS with CD14. This in turn inhibits the activation of the NF-κB signaling pathway and the subsequent release of inflammatory mediators

Article Snippet: After electrophoresis separation, the proteins were transferred to the membrane, which was blocked with 5% skim milk for 2 h. Subsequently, the membrane was incubated overnight at 4 °C with the primary antibodies against OPG (1:1000; Affinity Bioscience, OH, USA), RANKL (1:3000; Proteintech Group, Inc, Wuhan, China), RANK (1:500; Abcam, Cambridge, UK), TLR4 (1:500; Proteintech Group, Inc), and β-actin (1:10000) (Tiandeyue (Beijing) Biotechnology Co., Ltd, China).

Techniques: Binding Assay, Activation Assay

Journal: Frontiers in Microbiology

Article Title: Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis

doi: 10.3389/fmicb.2024.1320500

Figure Lengend Snippet: The effect of Shengu granules on intestinal barrier, FOXP3 cells and OPG/RANKL Signaling pathway protein expression. The colon pathological sections of the Sham, OVX and SG group, the former one (magnification, 40X), the latter one (magnification, 200X) (A) . Necrosis and exfoliation of mucosal epithelial cells (brown arrow), lymphocyte infiltration (red arrow), and nuclear fragmentation (black arrow) in Sham group; Nuclei were fragmented (black arrow), and more lymphocytes were found in the lamina propria (red arrow) in OVX group. Small focal infiltration of lymphocytes (red arrows) in SG group (A) . The Immunofluorescence results of each group (magnification, 200X) (B) . Histological score (C) . Percentage of mean Immunofluorescence intensity of FOXP3 cells in the three groups (D) . Protein blot analysis, semiquantitative analysis of protein blotting results of ZO-1 and Occludin in colon biopsy, OPG and RANKL in tibia tissue (E) . The ZO-1 and Occludin protein expression in colon tissue (F,G) . The OPG and RANKL protein expression ratio (H) . The OPG and RANKL protein expression in tibia tissue (I,J) . Statistical significance was evaluated using one-way ANOVA procedure and Tukey test. Different letters represent significant differences between groups ( p < 0.05) vs. OVX group. n = 5, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are represented as mean ± SD.

Article Snippet: Total protein samples extracted from colon tissue were incubated with ZO-1 (Servicebio Technology Ltd., China) and Occludin (Servicebio Technology Ltd., China) primary and secondary antibodies, and total protein samples extracted from femur tissue were incubated with OPG (Servicebio Technology Ltd., China) and RANKL (Servicebio Technology Ltd., China) primary and secondary antibodies.

Techniques: Expressing, Immunofluorescence